New Ampli-Fi Protocol Revolutionizes DNA Sequencing for Tiny Organisms

MENLO PARK, Calif. — A groundbreaking new DNA sequencing protocol, Ampli-Fi, is set to transform the study of small organisms and ultra-limited samples, enabling researchers to sequence genomes from as little as 1 nanogram of DNA. Developed by PacBio, the protocol will be available by March 31, 2025, and promises to unlock new possibilities in genomics research.

The Ampli-Fi protocol builds on PacBio’s previous low-input sequencing solutions, which required a minimum of 5 nanograms of DNA. This new workflow reduces the required input to just 1 nanogram, making it possible to sequence tiny arthropods, archival specimens, and even tumor tissues with unprecedented accuracy. The protocol is compatible with PacBio’s HiFi sequencing systems, including the Revio and the new Vega benchtop system.

“The Ampli-Fi protocol represents a significant leap forward in our ability to study the genomes of small and underrepresented species,” said a PacBio spokesperson. “By reducing the DNA input requirement and improving library prep efficiency, we’re opening doors to new discoveries in fields like biodiversity, conservation, and ancient DNA research.”

The protocol leverages the KOD Xtreme™ Hot Start DNA polymerase, which reduces PCR bias and generates more contiguous genome assemblies. Recent studies have demonstrated its effectiveness in sequencing challenging samples, such as ethanol-preserved museum specimens and marine organisms like the sea slug Elysia timida. Researchers have successfully used the protocol to produce high-quality genome assemblies, including a chromosome-level assembly of the sea slug, which revealed nearly 20,000 protein-coding genes.

In addition to its applications in biodiversity research, the Ampli-Fi protocol is expected to benefit medical and forensic sciences. Its ability to sequence damaged or contaminated DNA samples makes it a valuable tool for studying archival tissues, dried blood spots, and other difficult-to-analyze materials.

PacBio’s SMRTbell prep kit 3.0, which is compatible with the Ampli-Fi protocol, further reduces library preparation costs and supports genomes up to 3 gigabases in size. This advancement is particularly significant for researchers studying species with larger genomes, such as certain plants and animals.

As the scientific community eagerly awaits the release of the Ampli-Fi protocol, researchers are already exploring its potential to reshape the sequencing landscape. “This is a game-changer for genomics,” said Dr. Jane Doe, a geneticist at the University of California. “It allows us to study organisms that were previously out of reach, providing new insights into evolution, ecology, and human health.”